WheatBarrier™ Pathway

Wheat Immune Reactivity • Intestinal Barrier Integrity
A targeted immune-informed approach to wheat sensitivity and gut barrier dysfunction.

(U.S. exclusive)

Overview

Wheat-related immune responses rarely exist in isolation. They are often amplified or made clinically relevant by compromised intestinal barrier integrity.

WheatBarrier™ combines two foundational Cyrex panels to assess both immune reactivity to wheat and the intestinal permeability pathways that allow those reactions to persist.

This pathway helps clinicians move beyond surface-level wheat sensitivity testing to understand why immune activation is occurring and how to address it more effectively.

Why WheatBarrier™

Many patients remove gluten yet continue to experience symptoms. In these cases, the missing piece is often barrier dysfunction.

WheatBarrier™ is designed to answer three critical clinical questions:

  • Is the immune system reacting to wheat proteins including processed forms found in real-world diets?
  • Is intestinal permeability present and through which pathways?
  • Could barrier breakdown be perpetuating immune activation, inflammation, or autoimmunity?

By pairing immune reactivity with barrier assessment, WheatBarrier™ delivers context not just data.

What's Included with WheatBarrier™ Pathway

WheatBurden™

Immune Reactivity to Wheat, Gluten, and Their Processed Protein Forms
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Array 2

Intestinal Antigenic Permeability Screen™
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WheatBurden™ (Formerly Array 3X)

Immune Reactivity to Wheat, Gluten, and Their Processed Protein Forms

Detects immune reactivity to the full spectrum of wheat and gluten proteins, including processed wheat protein forms commonly consumed in real-world diets.

This expanded approach reflects how wheat exposure actually occurs and not just as raw flour or isolated gluten fractions.

What It Measures:

  • Multiple gliadins and glutenins
  • Wheat germ agglutinin (WGA)
  • Wheat albumins and globulins
  • Native and deamidated gliadin peptides
  • Gliadin–transglutaminase complexes
  • Microbial and tissue transglutaminases
  • Immune responses to processed wheat products (e.g., breads, doughs, pasta)

Helps determine whether wheat is a primary immune driver contributing to broader food sensitivity patterns.

Array 2

Intestinal Antigenic Permeability Screen™

Immune-Based Assessment of Gut Barrier Integrity

Array 2 evaluates immune responses associated with key intestinal permeability pathways, providing insight into whether barrier breakdown may be driving immune exposure.

Measures IgG, IgA, and IgM antibodies to:

  • Actomyosin - structural integrity of the epithelial barrier
  • Occludin/Zonulin - tight junction regulation
  • Lipopolysaccharides (LPS) - translocation of endotoxins

This immune-based permeability assessment helps contextualize food and environmental immune reactivity findings.

Clinical Application

The WheatBarrier™ Pathway is designed for patients with symptoms or histories suggestive of wheat sensitivity, immune dysregulation, or barrier dysfunction.

Ideal for patients with:

  • Persistent symptoms despite gluten avoidance
  • Chronic bloating, GI discomfort, or IBS-like presentations
  • Autoimmune or pre-autoimmune patterns
  • Brain fog, fatigue, or systemic inflammation
  • Dermatologic or joint symptoms of unclear origin
  • History of stress, infection, medication use, or environmental exposure

How to use WheatBarrier™ in Practice

  1. Assess the barrier first. Array 2 identifies whether intestinal permeability is present and which pathways are involved.
  2. Evaluate true wheat immune activation. WheatBurden™ clarifies whether immune responses target raw, modified, or processed wheat proteins.
  3. Connect exposure to tolerance loss. When barrier integrity is compromised, immune responses to wheat antigens become more clinically significant.
  4. Guide smarter dietary and clinical decisions. Results help inform targeted dietary strategies, barrier support, and monitoring over time.

    Why This Approach Matters

    The Wheat immune reactivity without barrier context is incomplete.

    Barrier dysfunction without antigen specificity lacks direction.

    WheatBarrier™ integrates both to deliver a clearer clinical roadmap for complex GI and immune presentations.