WheatBurden™
(Formerly Array 3X)
Immune Reactivity to Wheat, Gluten, and Their Processed Protein Forms
The full picture of wheat-driven immune reactivity.
A full-spectrum wheat/gluten immune assessment that detects reactivity, toxic peptides, barrier-related impact, and transglutaminase autoimmunity—immune activity that routine gliadin and tTG-2 screening may miss.
WheatBurden™
A broader view than standard gluten testing.
Traditional celiac work-ups focus on a narrow set of gliadin and transglutaminase-2 antibodies. WheatBurden™ expands this view across multiple wheat proteins, toxic peptides, food opioids, non-gluten proteins, and several transglutaminase enzymes—capturing early or atypical immune activity that routine tests may overlook.
Clarify wheat-related diagnoses
Differentiate non-celiac gluten sensitivity, Celiac disease, and other wheat-related autoimmunity by measuring IgG and IgA antibodies against a spectrum of antigens within the wheat/gluten proteome.
Assess systemic impact
Identify food opioid reactivity and transglutaminase-related autoimmunity that may contribute to fatigue, pain, skin findings, mood changes, or neuro-autoimmune presentations beyond the gut.
Guide what comes next in care
Use pattern-based interpretation to justify strict gluten removal, inform gut-support protocols, and decide when to add cross-reactive food panels or predictive autoimmunity testing within the Cyrex System.
What this profile measures
Antigens tested (IgG and IgA measured separately for each antigen)
WheatBurden™ evaluates IgG and IgA antibodies against a comprehensive set of wheat, gluten, and enzyme targets. Idenitfy immune reactivity across wheat components, gluten peptides, and cross-reactive enzyme markers.
Antigens List
Full Wheat Kernel
- Wheat
- Wheat Germ Agglutinin
Non-Gluten Wheat Proteins
- Non-Gluten Proteins - A
- Non-Gluten Proteins - B
Gluten Family Peptides
- Gliadin Toxic Peptides
- Native + Deamidated Alpha-Gliadin-33-mer
- Alpha-Gliadin-17-mer
- Gamma-Gliadin-15-mer
- Omega-Gliadin-17-mer
- Glutenin-21-mer
- Gluteomorphin + Prodynorphin
Gliadin Transglutaminase
- Gliadin-Transglutaminase Complex
Transglutaminase
- Microbial Transglutaminase
- Transglutaminase-2
- Transglutaminase-3
- Transglutaminase-6
Cooked/Processed Wheat Products
- White Flour Dough
- Whole Wheat Bread (Yeast-Free)
- Sprouted Whole Wheat Bread
- Wheat Tortilla Bread (Yeast-Free)
- American Sourdough Bread
- Italian Wheat Bread
- Italian Pasta (Cooked)
Illustration dramatized for visual purposes. © Cyrex Laboratories, LLC. All rights reserved.
How WheatBurden™ fits within the Cyrex System
WheatBurden™ serves as the cornerstone wheat/gluten panel in the React category of the Cyrex System. It can also inform predictive and barrier-focused strategies when combined with other panels, such as:
- Array 4 when you need to evaluate non-wheat foods that may perpetuate gluten-like immune responses.
- Array 5 when transglutaminase reactivity suggests evolving autoimmunity or multi-organ involvement.
- The Lymphocyte MAP™ when you want a broader view of immune balance, loss of tolerance, and systemic inflammatory burden.
Clinical Application
When to use WheatBurden™
Use WheatBurden™ when you suspect wheat/gluten may be contributing to chronic symptoms involving the gut, nervous system, or skin—yet standard celiac labs are negative, incomplete, or do not fully explain the patient's clinical presentation.
- Persistent GI complaints where basic dietary elimination has not resolved symptoms.
- Suspected Celiac disease, non-celiac gluten sensitivity, dermatitis herpetiformis, gluten ataxia, or other wheat/gluten-related disorders.
- Autoimmune risk or family history where wheat- related autoimmunity could be an upstream driver.
- Non-responsive GI symptoms in patients already following a gluten-reduced or gluten-free diet.
- Multiple symptom complaints (e.g., Chronic Fatigue Syndrome, Fibromyalgia) where food opioids and wheat reactivity may contribute to systemic burden.
- Depression, cognitive changes, or neuro-autoimmunity where gluten involvement is suspected.
Contact Us
If you would like to learn more about the
WheatBurden (Formerly Array 3X) — Immune Reactivity to Wheat, Gluten, and Their Possible Protein Forms.
WheatBurden (Formerly Array 3X) — Immune Reactivity to Wheat, Gluten, and Their Possible Protein Forms.
To Get Started
Cyrex Laboratories works with licensed healthcare professionals throughout the United States.
Create a healthcare professional account now!
If you're already a Cyrex Labs account holder, click here to log into your account to download additional materials.
Create a healthcare professional account now!
If you're already a Cyrex Labs account holder, click here to log into your account to download additional materials.